Molecular characterization and in vitro control measures of fruit rot disease of Sweet pepper

Fruit rot disease of sweet pepper is one the main fungal diseases causing huge economic losses to grower. An experiment was conducted find out pathogen associated with fruit pepper, obtained from experimental fields Jahangirnagar University, Bangladesh. disease-causing fungus isolated infected fruits and identified using morphological characterization based on colony features, mycelia, conidia as well molecular internal transcribe spacer (ITS) region fungus. ITS sequence our studied MH368146.1 genetically 99-100% similar sequences Fusarium solani in NCBI database. Typical symptoms were reproduced by artificial inoculations The mycelial growth this evaluated ten different solid culture media i.e., Potato Dextrose Agar, Yeast Extract Honey Peptone Hansen’s Medium, Sabouraud’s Glucose Kauffman’s Sucrose Richard’s Agar Carrot Agar. Fungus grew all tested media. Several bio-control agents two commercial fungicides against under vitro condition, which highest percent inhibition radial determined 64.75% due Trichoderma reesei isolate 2, 60.63% Tilt 250 EC (500 ppm) at 7 days post-incubation. Therefore, T. found most suitable control F. laboratory conditions. However, further pot field trials needed be confirmed potential it. Int. J. Agril. Res. Innov. Tech. 11(2): 108-116, Dec 2021